List the steps of preparation of temporary mount of a leaf peel to observe stomata.

To prepare a temporary mount of a leaf peel to observe stomata, follow these steps:

1. Peeling: Gently bend/fold a freshly plucked leaf (such as a Rheo or Tradescantia leaf) and pull a thin, translucent peel from its lower surface using a pair of forceps.
2. Transferring: Place the leaf peel immediately into a watch glass containing clean water to prevent it from drying out.
3. Staining: Transfer the peel to a watch glass containing a few drops of Safranin stain for about 1–2 minutes to color the cell walls and nuclei.
4. Mounting: Place the stained peel on a clean glass slide. Add a drop of glycerine over it to prevent drying, and gently place a coverslip over the peel using a needle at an angle to avoid trapping any air bubbles. Absorb excess stain/water using filter paper.
5. Observation: Observe the slide under the low power of a microscope first, and then switch to high power to clearly see the stomatal pores and guard cells.